Screening of Transgenic Plants by Amplification of Unknown Genomic DNA Flanking T-DNA
نویسندگان
چکیده
منابع مشابه
Screening of transgenic plants by amplification of unknown genomic DNA flanking T-DNA.
For the screening of transfer DNA (T-DNA) integration in transgenic plant material, we developed a method based on specific amplification of genomic plant DNA flanking T-DNA borders. This approach is possible because the length of the region flanking T-DNA extremity on a restriction fragment is specific to the integration locus. We have modified an adaptor ligation PCR technique developed for a...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99272st01